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Streptavidin (bs-0437P)  
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說明書: 1mg  5mg  10mg
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產(chǎn)品編號 bs-0437P
英文名稱 Streptavidin
中文名稱 鏈霉親和素
別    名 SA V1; SA V2; Streptavidin V1; Streptavidin V2; SA.  鏈親和素; Streptavidin 鏈霉親和素;
Specific References  (18)     |     bs-0437P has been referenced in 18 publications.
[IF=14.188] Xuehui Liu. et al. Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers. 2021 Dec 19  
[IF=10.334] Zhao Cui. et al. Chip-DSF: A rapid screening strategy for drug protein targets. PHARMACOL RES. 2022 Aug;182:106346  
[IF=8.008] Li Fu. et al. A General Route for Chemiluminescence of n-Type Au Nanocrystals. ANAL CHEM. 2022;94(24):8811–8817  IA ;  
[IF=8.008] Yi Ma. et al. Flap Endonuclease-Induced Steric Hindrance Change Enables the Construction of Multiplex and Versatile Lateral Flow Strips for DNA Detection. ANAL CHEM. 2022;94(42):14725–14733  Other ;  Other.  
[IF=6.911] Zhuoer Zeng. et al. Nonlinear hybridization chain reaction-based flow cytometric immunoassay for the detection of prostate specific antigen. ANAL CHIM ACTA. 2022 Aug;1220:340048  
[IF=6.14] Wenhao Li. et al. Biotinylated Au Nanoparticle-Based Artificial Antibody for Detection of Lysozyme by the Lateral Flow Immunoassay and Enzyme-Linked Immunosorbent Assay. ACS APPL NANO MATER. 2022;XXXX(XXX):XXX-XXX  Other ;  Other.  
[IF=5.977] Jing Chen. et al. A novel method combining aptamer-Ag10NPs based microfluidic biochip with bright field imaging for detection of KPC-2-expressing bacteria. Anal Chim Acta. 2020 Oct;1132:20  Other ;  
[IF=5.44] Zhang, Juan, et al. "Amplified amperometric aptasensor for selective detection of protein using catalase-functional DNA-PtNPs dendrimer as a synergetic signal amplification label." Biosensors and Bioelectronics (2014).  other ;  
[IF=5.221] Yang Guo . et al. Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide. FRONT CHEM. 2022; 10: 898924  
[IF=5.01] Dong, Peipei, et al. "Ultrathin Gold-Shell Coated Silver Nanoparticles onto a Glass Platform for Improvement of Plasmonic Sensors." ACS Applied Materials & Interfaces (2013).  Other ;  
[IF=4.57] Pu, Caixiu, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles for the Treatment of Intraperitoneal Ovarian Cancer Xenografts." Molecular pharmaceutics (2013).  other ;  Human.  
[IF=4.57] Liu, Hongxia, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles Induces Proliferation Inhibition and Apoptosis in Ovarian Cancer Cells." Molecular Pharmaceutics (2013).  other ;  Biotin.  
[IF=4.162] Houren Zhou. et al. “PFH/AGM-CBA/HSV-TK/LIPOSOME-Affibody”: Novel Targeted Nano Ultrasound Contrast Agents for Ultrasound Imaging and Inhibited the Growth of ErbB2-Overexpressing Gastric Cancer Cells. DRUG DES DEV THER. 2022; 16: 1515–1530  
[IF=3.55] Huang, Mo, et al. "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." Colloids and Surfaces B: Biointerfaces 85.1 (2011): 92-96.  Other ;  
[IF=3.54] Chen, Feifei, et al. "Direct growth of coupled gold nanoparticles on indium tin oxide substrate and construction of biosensor based on localized surface plasmon resonance." Sensors and Actuators B: Chemical (2013).  other ;  Biotin.  
[IF=3.532] Zhaokui Zeng. et al. Rational design of nonlinear hybridization immunosensor chain reactions for simultaneous ultrasensitive detection of two tumor marker proteins. ANAL METHODS-UK. 2023 Feb;:  
[IF=3.43] Jiang, Yixuan, et al. "Sensitive aptamer-based fluorescence polarization assay for mercury (II) ions and cysteine using silver nanoparticles as a signal amplifier." Microchimica Acta (2014): 1-8.  Other ;  
[IF=3.375] Yun Liu. et al. Synergistic anti-tumor effect of anti-PD-L1 antibody cationic microbubbles for delivery of the miR-34a gene combined with ultrasound on cervical carcinoma. Am J Transl Res. 2021; 13(3): 988–1005  Other ;  
理論分子量 12kDa
檢測分子量 12/38/55
性    狀 Lyophilized
物    種 Streptomyces
序    列 25-183/183
純    度 >95% as determined by SDS-PAGE
純化方法 AC
內(nèi)毒素 Not analyzed
表達系統(tǒng) N/A
活性 Yes
標簽 Tag free
緩 沖 液 Lyophilized from 0.22um filtered solution in PBS (pH7.4).
保存條件 Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產(chǎn)品介紹 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

SWISS:
P22629

Gene ID:
N/A


鏈霉親和素(Streptavidin,SA)產(chǎn)品簡介
鏈霉親和素具有與親和素(Avidin,AV)相似的生物學特性,如都能與4分子的生物素(Biotin)特異性結合,結合常數(shù)為1×10-15/M。同時,由于SA不帶糖基、等電點低,在檢測中具有比AV低的背景而大大提高了檢測的靈敏性。
生物素與親和素/鏈霉親和素的結合具有高度特異性和穩(wěn)定性,兩者的親和常數(shù)比抗原-抗體反應高10-100萬倍,是目前已知強度最高的非共價作用,這使得它成為免疫學檢測中的一個非常好信號放大的工具。廣泛應用于酶聯(lián)免疫反應、熒光分子標記免疫反應和分子雜交為原理的各種檢測試劑盒的SA-biotin系統(tǒng)。但目前,國內(nèi)所用SA均為價格昂貴的進口產(chǎn)品,因此研制和生產(chǎn)國產(chǎn)化的SA具有重要的現(xiàn)實意義和廣闊的應用前景。
????鏈霉親和素是與親和素具有相似性質(zhì)的一種蛋白質(zhì),都具有與生物素特異結合的能力,結合常數(shù)高達1015M。同時,由于鏈霉親和素等電點比親和素低,且不含糖基鏈,故在檢測中的靈敏度和特異性都高于親和素,建立在此基礎上的生物素-鏈霉親和素系統(tǒng)的應用比生物素-親和素系統(tǒng)有更大優(yōu)勢。
????我公司生產(chǎn)用鏈霉親合素-SA菌株及生產(chǎn)工藝均自國外原本帶回。生產(chǎn)工藝穩(wěn)定,保證質(zhì)量,常年提供產(chǎn)品,確保供應。
我公司生產(chǎn)的SA單體經(jīng)SDS-PAGE檢驗分子量為17.2KDa。經(jīng)論證,完整的SA溶解性差、易于聚集,核心SA被蛋白酶水解掉了與活性無關的某些末端序列,一方面提高了結構的穩(wěn)定性;另一方面由于減少了空間位阻,使SA更容易與生物素標記的大分子發(fā)生反應。
SA的純化是用2-亞氨基生物素瓊脂糖珠4B 凝膠(Sigma 產(chǎn)品)進行親和層析純化,同時以離子交換進行進一步的精細純化。
SA分子量為58KDa,生物活性15.7U/mg,接近理論值(16.8U/mg)并進行了活性測定。
SA溶解與保存:我公司提供的SA產(chǎn)品,不含任何鹽離子,用戶可以根據(jù)自己的試驗需要,使用不同的緩沖液進行溶解,溶解后應放與-20°C保存,避免反復凍溶。

產(chǎn)品圖片
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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